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![]() As a multi-purpose concentration gradient maker, it has four independent inlets. In this work, we describe a device fabricated by soft lithography and consisting of a Christmas-tree network. Thus, it may be necessary to redesign a footprint if applied to new occasions. The concentration distribution profile within the channel is determined by the length of the channel, the splitting ratio of the fluids, and also the numbers of inlets and outlets. They have been widely studied in various fields in recent decades. Microfluidic concentration gradient generators are among the most efficient devices employed for precise generation of various concentration gradients. By the combination control of flow rate and chip geometries, an automated drug dilution platform was presented recently. Another principle of structure-based mixing or concentration gradient makers is topological mixing schemes, which split, rotate, and recombine the microflows to perform efficient mixing by diffusion. By varying the lengths of microchannel, the volume of dispensed sample solutions was dictated, and then complex gradient profiles were generated. One more example, a structure-based approach, was presented to generate linear or nonlinear chemical gradients. In this work, it was shown that the concentration gradient profile could be controlled not only by the dynamic inputs but also by channel geometries. This approach has been successfully used to mix many kinds of microfluids. In addition, a systematic investigation was carried out to demonstrate the universal approach to generate stable gradients of any profiles by experimental and mathematical methods. By using this device, linear gradients with dynamically controlled characteristics (i.e., slope, baseline, or direction of the line), and nonlinear gradients with controlled nonlinearity were illustrated. Then, another impressive device was developed to generate dynamic temporal and spatial concentration gradients using one single microfluidic device. One of the earliest designs was a design for controlled diffusive mixing of reagents, which generated a range of shapes for the gradients, such as linear, parabolic, and periodic. To address a nonlinear concentration gradient profile, a range of different microfluidic mixing technologies have been developed. Generally speaking, a linear concentration gradient generator can be easily set up by classic Christmas-tree microfluidic channel networks, convection-driven flow, or novel designs such as centrifugal microfluidics or 3D-printing stereo networks. Past research studies have shown that a device that can generate spatially and temporally controlled gradients is regarded as a robust and powerful method to investigate migratory cells, drug screening, oil production screening from microalgae, nucleation and growth of crystals, and wound healing under a variety of conditions. ![]() In fact, the ability to generate stable linear or nonlinear spatial chemical gradient profiles within microfluidics has been adapted extensively for analysis. With the help of external resources, such as electric signals, magnetic fields, acoustic energy, or light sources, microfluids can be mixed efficiently. Most biological or chemical samples are composed of a mixture of several kinds of reagents, and thus on-chip mixing is an essential step in many applications. ![]() With their advantages of low reagent consumption, large integration in small footprints, near-zero dead volume, and low-cost fabrications, polydimethylsiloxane (PDMS) microfluidic devices are increasing used for lab-on-a-chip applications. With on-demand flow rate ratios, the FFRR microfluidic device could be used for many lab-on-a-chip applications where flexible concentration profiles are required for analysis. Its performance was analyzed using numerical simulation models and experimental investigations, and it showed an excellent time response (~10 s). ![]() To demonstrate the advantages of this approach, we used a Christmas-tree-like microfluidic chip as the demo. Here, we present an FFRR (feed flow rate ratio) adjustment approach to generate tens of types of concentration gradient profiles with one single device. To address this challenge, we developed a soft-lithography-fabricated microfluidic platform that enabled one single device to be used as a concentration maker, which could generate linear, bell-type, or even S-type concentration profiles by tuning the feed flow rate ratios of each independent inlet. These microfluidic devices have untapped potential for varying concentration patterns by the use of one single device or by easy-to-operate procedures. Microfluidic chips-in which chemical or biological fluid samples are mixed into linear or nonlinear concentration distribution profiles-have generated enormous enthusiasm of their ability to develop patterns for drug release and their potential toxicology applications. ![]() ![]() ![]() Roth and the group then beat Hendricks and hang him from a tree. During this, Hendricks accidentally drops his medicine bottle, and it is run over by a car. Roth calls Catherine and several others and they kidnap Hendricks. One night, while Roth's teenage daughter, Kelly, is out, a fearful Roth jumps to the conclusion that Hendricks has taken her. Many people are not happy about Hendricks's release, especially an activist group led by Jackson Roth and Catherine "Sunny" Macintosh. While taking his medicine, Hendricks is timid and apologetic about what he did, but the memories of what Hendricks did are still fresh in the minds of Helverton's residents. So, Hendricks moves back to his old neighborhood. Doctors at the Meistrich Institute state that Hendricks, who has been diagnosed as a schizophrenic with a severe chemical imbalance, is okay as long as he is on his medication. But a year later, Hendricks is declared not guilty by reason of insanity and he is put in the Meistrich Psychiatric Institute, only to be released three years later. After a brief struggle in which Captain Howdy gets shot, Mike arrests him and discovers his real name is Carlton Hendricks. There, Gage finds Genevieve naked and bound, with her mouth stitched shut, as well as five other teenagers who are in similar predicaments. Despite the plan going awry, he later figures out Captain Howdy's location and finds his torture chamber. ![]() Meeting the Captain Howdy online, Mike attempts to get Captain Howdy to invite him to a party. But it is not until Mike's niece Angela Stravelli informs him of Genevieve's penchant for meeting strangers through the Internet that Mike gets his first lead. Mike discovers that Captain Howdy is into "body art," including significant tattooing, piercing, branding, and scarification. The case takes an unexpected turn when Tiana's car is pulled out of a lake with Tiana's tortured body inside and no sign of Genevieve. With the assistance of a younger cop named Steve Christian, Gage begins searching for Genevieve and Tiana. When neither Genevieve nor Tiana returns home by the next morning, Genevieve's mother, Toni, alerts her husband, local cop Mike Gage. After chatting with another apparent student who goes by the screen name of "Captain Howdy," Genevieve and Tiana decide to attend a party at Captain Howdy's house, which is a trap. Accordingly, a large amount of dialogue of the film's villain (concerning his personal philosophy) are paraphrases or direct quotations of Fakir Musafar, the father of the Modern Primitive movement.įifteen-year-old Genevieve Gage and her best friend Tiana Moore are typical high school students in Helverton, Colorado who spend their idle time chatting with strangers in chat rooms. The film has a strong emphasis on the Modern Primitive subculture and its ethos of spiritual transcendence through painful rites, showing several such different practices therein. The film centers around a police detective trying to save his city, as well as his daughter, from an online predator who enjoys bringing "enlightenment" through ritual pain. Strangeland is a 1998 American horror film written by Dee Snider and directed by John Pieplow. ![]() ![]() What is perhaps one of the best Mario Bros games in history, was released for Nintendo 64 under the name Super Mario 64.
![]() ![]() ![]() Subscriptions for all services are for a pre-paid 15-month subscription term. Offer valid only on select PCs when bundling (includes Performance Guard Warranty and Microsoft 365 Personal and Family). Available only in Microsoft stores in the United States (excluding Puerto Rico) and Canada. Offer valid from September 23rd, 2020 through March 31st, 2022 while supplies last. File claim online through, with an Am Trust Phone Agent (888) 270-8432, or in a Microsoft Retail Store through a service advisor. A $99 deductible applies to any ADH claim or at any time when the customer is choosing to replace their device (During the OEM warranty period). One (1) ADH claim or up to three (3) repairs up to original purchase price. **Accidental Damage from Handling (ADH) is limited to damage resulting from dropping the covered product, spilling liquid onto it or screen/glass breakage of product. Get 15 months of Microsoft 365 for the price of 12. Certain features require specific hardware (see Windows 11 Specifications). See applicable Microsoft Terms of Sale for more information. Microsoft Store return policy applies to extended returns. Microsoft reserves the right to modify or discontinue offers at any time. Void where prohibited or restricted by law. Extended return offer period limited to 5 device returns total per eligible customer. Not available for purchases by reseller customers. Return process must be started within 60 days after customer received the device. *Extended return offer period available with Surface devices purchased from Microsoft Store in select markets. ![]() ![]() ![]() ![]() ![]() Copyright © 2017 Youthstream - All rights reserved. MXGP3 - The Official Motocross Videogame © 2017 Published and Developed by Milestone S.r.l. One-time license fee for play on account’s designated primary PS4™ system and other PS4™ systems when signed in with that account. The better you do in career mode - the more credits you will earn. Download the exclusive MXGP3 - Credits Multiplier additional content Multiply your credits after every race and in every game mode to reach the highest step. Download the exclusive MXGP3 - Credits Multiplier additional content Multiply your credits after every race and in every game mode to reach the highest step of the podium as quickly as possible In order to play with this additional content, it is necessary to first purchase game disc or full game from PlayStationStore that is sold. Online features require an account and are subject to terms of service and applicable privacy policy (/terms-of-service & /privacy-policy). You earn credits by completing races and finishing in higher positions. Software subject to license (us./softwarelicense). Network Players 2-12 - Full game requires PlayStation®Plus membership to access online multiplayer Online features require an account and are subject to terms of service and applicable privacy policy (/terms-of-service & /privacy-policy). Remote Play requires PS Vita system and sufficiently robust Wi-Fi connection. Download the exclusive MXGP3 - Credits Multiplier additional content! Multiply your credits after every race and in every game mode to reach the highest step of the podium as quickly as possible! 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![]() Do you need home design and landscape design rolled into one?įinally, if you need some inspiration for your new garden or yard, Pinterest is a great place to look at how other people have redesigned their home landscapes.Do you need to be able to easily share your yard plans with others?.Can the app show you how plant growth will look years down the line?.Can the software project how changes in seasons, sunlight and shadows will affect your design?.Are there plenty of quick start templates to customize and help get things done quickly?.Does the app have a detailed database of plants, shrubs and other garden related objects?.Do you need a sprinkler system layout planner built-in?.Do you want to be able to do 3D walk-throughs of your finished designs?.Do you need the software to pull GPS coordinates for contractors to view?.Do you want to be able to upload a photo of your home or garden?.Do you want a 2D or 3D landscaping tool?.To help you decide which landscaping app to go for, there are a few useful things to consider before diving in. What To Look For In The Best Landscape Design Software What To Look For In The Best Landscape Design Software.The Best Landscape Design Apps For iPhone, iPad and Android.The Best Home Design & Floor Plan Software.In our extensive research we found the best landscaping design software was SmartDraw for its ease of use and flexibility on all platforms. We’ve reviewed the best easy to use landscaping tools that can be used on all platforms that allow your imagination to run wild whether its flagstone patios, swimming pools, a vegetable patch or exotic flowers you fantasize about.Īlthough many of the most popular professional landscaping software like VizTerra, Lands Design and Realtime Landscaping Pro are for Windows only, there are also some excellent alternatives on Mac that can be used on Monterey, Big Sur, Catalina and the latest M1 & M2 Macs. ![]() ![]() The do-it-yourself route also puts you in a full control and nowadays you don’t need to use expensive CAD software to design landscapes.Ĭreating your own landscape plan also helps you to be more realistic when it comes to estimating the scale of renovations and costing for the materials involved. The average landscape project will easily set you back a few thousand dollars although this can easily rise depending on the size and details of your garden or project. Using landscape planning software can save you a fortune compared to the cost of hiring a professional landscape designer so we reviewed the best landscape design software for Windows & Mac of 2023. ![]() ![]() In their wild habitats, guppies live in moving water. Can Guppies Live With Platies? (+ How To Keep Them Together).Do Guppies Eat Bloodworms? (Live, Frozen, Or Freeze-Dried?).Do Guppies Lay Eggs Or Give Live Birth? (Explained Simply).Do Guppies Need A Filter? + How To Keep Guppies Without A Filter?.The optimum tank setup for guppies should have at least two gallons of water per guppy.Īpart from having enough water in the tank, proper filtration, aeration, and water flow will also prevent your guppies from blowing bubbles. You can prevent your guppies from being affected by ensuring an ideal tank setting in the first place. ![]() If you don’t have air stones or pumps in the aquarium, your fish will struggle to get oxygen. Poor tank maintenance and absence of aeration will further aggravate the issue. The severity of the problem increases if there are large fish that need more oxygen. So, too many tank mates can deprive the guppy of oxygen. This is because all the fish depend on the same oxygen source in a tank. In an overcrowded tank, guppies will struggle to meet their survival needs. More importantly, it limits their access to resources and clean water. Keeping guppies in an overcrowded tank can impact their health. Avoid overcrowding.Īlthough guppies are small, they need enough space and water to swim. Upgrade the filter if necessary to control ammonia spikes and the accumulation of other toxins. Use a proper testing kit to monitor the toxin levels in your tank regularly. Ensure that your tank filter is operating efficiently. You can prevent this problem by performing regular water changes. If the problem persists, their activity levels will reduce. Therefore, you can suspect low oxygen levels if you find them frequenting this space.Ĭontinuous deficiency of oxygen can make your guppies tired and sluggish over time. If your guppies cannot get enough oxygen, they will hang out in the upper areas of the tank. Insufficient oxygen will affect the overall health and well-being of your fish. Poor tank maintenance will also cause the oxygen levels to deteriorate. When their gills are impacted, the fish cannot breathe properly, and they produce bubbles. Increased ammonia levels will burn their gills and cause respiratory distress. Now, guppies are very sensitive to high ammonia levels. If your filter is inefficient, this process will be compromised. The main purpose of a filter is to remove the organic waste in a tank before it breaks down into poisonous ammonia and nitrates. ![]() Toxins will quickly build up in a poorly maintained tank. If you don’t regularly clean your filter or change the tank water, the water quality will deteriorate. The water quality and water chemistry of a tank depend on filtration and general tank maintenance. Given below are some steps you can take to stop guppies from blowing bubbles. If everything is under control, you should look at any problems that may impact guppies’ gills.īy addressing the underlying cause, you can stop your guppies from blowing bubbles. Look for likely issues that may cause low oxygen concentration in their tank. To stop your guppies from producing bubbles, you should know what is causing the problem. How To Stop Guppies From Blowing Bubbles? Can Guppies Live In Cold Water? (What Happens If You Do That?).How To Stop Guppies From Dying? (10 Different Ways).They rapidly inhale oxygen from the water and produce bubbles in the process. When ammonia burns their gills, they struggle to breathe. This commonly occurs in unhygienic tanks with high ammonia levels. Guppies will also produce bubbles if their gills are impacted and their breathing gets compromised. However, they will also end up producing bubbles. This helps them meet their oxygen requirements. When they don’t get enough oxygen, guppies will try to compensate by breathing rapidly. So they will be unable to breathe normally. ![]() Low oxygen concentrations can make them feel suffocated. Guppies usually blow bubbles when their breathing is impaired. However, if your guppies frequently blow bubbles, it deserves attention. It’s normal for most aquarium fish to expel bubbles once in a while. Should You Be Worried If Your Guppies Blow Bubbles? Let’s talk about this in greater detail now. They may also blow bubbles if their gills are impacted for some reason, and they are not able to breathe normally. When the oxygen concentration in the water is low, guppies tend to breathe rapidly and expel bubbles during the process. Guppies usually blow bubbles when they can’t breathe properly. So, if your guppies suddenly blow bubbles, you should suspect something is wrong. For instance, guppies don’t normally blow bubbles. However, sometimes weird traits may indicate serious problems. In most cases, their abnormal behavior is temporary. If you keep guppies, you may have noticed that they act weird at times. ![]() ![]() ![]() ![]() While, An issue that prevented the cleaning of Chrome’s download history also fixed now.Ġ3. A bug that was causing Easy Clean to disappear from the navigation menu fixed in the latestĠ2. Automatic Updates: Furthermore, It will automatically update once a month to stay on top.Ġ1. That are taking unnecessary and too much of space.ġ0. Finding Duplicate Files: While, It can also find the duplicate files and folders in your system Managing auto-starting program: Also, It prevents the programs to run automatically in theĠ9. While, It helps you get to work faster by letting you disable those programs that are not needed.Ġ8. 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